Date of Graduation
Summer 8-7-2020
Document Type
Thesis
Degree Name
Master of Science in Chemistry
College/School
College of Arts and Sciences
Department/Program
Chemistry
First Advisor
Ryan West
Abstract
In this thesis, electrochemical approaches are used to determine the properties of deoxyribonucleic acid (DNA) by electrostatic denaturation. The electrochemical routine involves an application of a destabilization potential, an equilibration potential, and a square wave voltammogram (SWV) to monitor the extent of melting. Our method uses a monolayer consisting of thiol modified DNA and mercaptohexanol on a gold electrode. These electrodes are then incubated in a complementary sequence tagged with methylene blue. By using our electrostatic denaturation technique, different parameters are explored, such as surface densities, surface coverages, and ionic strengths. As proof of concept, these techniques were applied toward detecting cisplatin interactions on a DNA surface. Depending on the surface coverage, cisplatin changes DNA stability accordingly.
Recommended Citation
Madrigal, Eddie, "Electrostatic Denaturation of DNA-Cisplatin Adducts" (2020). Master's Theses. 1326.
https://repository.usfca.edu/thes/1326
