Document Type
Article
Publication Date
2002
Abstract
Cohesion between sister chromatids is a prerequisite for accurate chromosome segregation during mitosis and meiosis. To allow chromosome condensation during prophase, the connections that hold sister chromatids together must be maintained but still permit extensive chromatin compaction. In Drosophila, null mutations in the orientation disruptor (ord) gene lead to meiotic nondisjunction in males and females because cohesion is absent by the time that sister kinetochores make stable microtubule attachments. We provide evidence that ORD is concentrated within the extrachromosomal domains of the nuclei of Drosophila primary spermatocytes during early G2, but accumulates on the meiotic chromosomes by mid to late G2. Moreover, using fluorescence in situ hybridization to monitor cohesion directly, we show that cohesion defects first become detectable in ord(null) spermatocytes shortly after the time when wild-type ORD associates with the chromosomes. After condensation, ORD remains bound at the centromeres of wild-type spermatocytes and persists there until centromeric cohesion is released during anaphase II. Our results suggest that association of ORD with meiotic chromosomes during mid to late G2 is required to maintain sister-chromatid cohesion during prophase condensation and that retention of ORD at the centromeres after condensation ensures the maintenance of centromeric cohesion until anaphase II.
DOI
10.1091/mbc.E02-06-0332
Recommended Citation
Balicky EM1, Endres MW, Lai C, Bickel SE. Meiotic cohesion requires accumulation of ORD on chromosomes before condensation. Mol Biol Cell. 2002 Nov;13(11):3890-900.
Comments
Copyright American Society for Cell Biology. Article available at: http://dx.doi.org/10.1091/mbc.E02-06-0332
Published under Creative Commons License 3.0 Unported: http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode
Errata/Correction from publisher: Due to a press error, several figures in the article “Meiotic cohesion requires accumulation of ORD on chromosomes prior to condensation” by E. Balicky, M. Endres, C. Lai, and S. Bickel (Mol. Biol. Cell [2002], 13, 3890–3900, 10.1091/mbc.E02-06-0332) were incorrectly reproduced in print and for the online PDF version. The correct figures can be viewed online at www.molbiolcell.org by accessing the full text (HTML) version of the published article.