Major
Biotechnology
Research Abstract
Jurkat T cells have been used as a model cell in CAR-T engineering for cancer research. T cells function in the immune response of the body to rid it of infections and disease. CAR-T is able to bind to cancerous cells and turn on the immune response to kill tumor cells. The goal of this experiment is to activate Jurkat T cells using CD3/CD28 beads and to measure activation by CD69 expression. Artificial activation was measured by CD69 antibody staining with phycoerythrin (PE) and analysis by flow cytometry. By analyzing activation of T cells, we are able to determine the sensitivity of its activation. Results will be used to help design and develop a more sensitive CAR-T therapy.
Faculty Mentor/Advisor
Cary Lai
Activation of Jurkat T Cells with CD3/CD28 beads and analysis by CD69 markers
Jurkat T cells have been used as a model cell in CAR-T engineering for cancer research. T cells function in the immune response of the body to rid it of infections and disease. CAR-T is able to bind to cancerous cells and turn on the immune response to kill tumor cells. The goal of this experiment is to activate Jurkat T cells using CD3/CD28 beads and to measure activation by CD69 expression. Artificial activation was measured by CD69 antibody staining with phycoerythrin (PE) and analysis by flow cytometry. By analyzing activation of T cells, we are able to determine the sensitivity of its activation. Results will be used to help design and develop a more sensitive CAR-T therapy.
