Antibody binding screens using Biolayer Interferometry (BLI) and Surface Plasmon Resonance (SPR)

BLI and SPR are both label-free technologies being used to measure biomolecular interactions in real-time. Distributed Bio uses computational immunology techniques to generate antibody libraries against a target and bio-panning to identify and select clones that best interact with the target. The interaction is further tested using BLI and SPR technology platforms that enable determination of binding kinetics such as association and dissociation rates also known as on-rates and off-rates. Analysis of the on-rate and off-rate data is further used to determine and report the binding affinity of the target to the antibody clones which are then delivered to the clients. As an intern at Distributed Bio, I am learning the BLI and SPR technology platforms to quality check target antigens used to select antibody clones, and to identify the highest binding clones to the target.