Date of Graduation
Winter 12-13-2013
Document Type
Thesis
Degree Name
Master of Science in Chemistry
College/School
College of Arts and Sciences
Department/Program
Chemistry
First Advisor
Dr. Megan E. Bolitho
Second Advisor
Dr. Lawrence Margerum
Third Advisor
Dr. Jie Jack Li
Abstract
Quorum sensing (QS) is a process of coordination of bacterial gene expression in response to cell population. System two QS is regulated by the small signaling molecule autoinducer-2 (AI-2) and is implicated in the infectious behaviors of various bacterial species. AI-2 is biosynthesized from S-ribosylhomocysteine (SRH) by the enzyme LuxS and induces interspecies cell-to-cell communication. Inhibition of LuxS would therefore inhibit interspecies QS. Herein, a search for novel molecular species that will competitively bind with SRH in the LuxS binding site is performed in silico. Computational screening results are then validated in vitro using an optimized LuxS inhibition assay.
Recommended Citation
Wang, Keeshia Q., "Biochemical Assay Optimization and Computational Screening Efforts to Identify Potential LuxS Inhibitors" (2013). Master's Theses. 149.
https://repository.usfca.edu/thes/149
Included in
Amino Acids, Peptides, and Proteins Commons, Biochemical Phenomena, Metabolism, and Nutrition Commons, Biochemistry Commons, Biotechnology Commons, Enzymes and Coenzymes Commons, Medical Biochemistry Commons, Other Biochemistry, Biophysics, and Structural Biology Commons, Other Chemicals and Drugs Commons, Pharmaceutical Preparations Commons
