Date of Graduation
Master of Science in Chemistry
College of Arts and Sciences
Dr. Megan E. Bolitho
Dr. Lawrence Margerum
Dr. Jie Jack Li
Quorum sensing (QS) is a process of coordination of bacterial gene expression in response to cell population. System two QS is regulated by the small signaling molecule autoinducer-2 (AI-2) and is implicated in the infectious behaviors of various bacterial species. AI-2 is biosynthesized from S-ribosylhomocysteine (SRH) by the enzyme LuxS and induces interspecies cell-to-cell communication. Inhibition of LuxS would therefore inhibit interspecies QS. Herein, a search for novel molecular species that will competitively bind with SRH in the LuxS binding site is performed in silico. Computational screening results are then validated in vitro using an optimized LuxS inhibition assay.
Wang, Keeshia Q., "Biochemical Assay Optimization and Computational Screening Efforts to Identify Potential LuxS Inhibitors" (2013). Master's Theses. 149.
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